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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
28/11/2018 |
Actualizado : |
28/11/2018 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
NÚÑEZ, A.; MORON, A. |
Afiliación : |
AGUSTIN NUÑEZ RUSSI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DAVID ALEJANDRO MORON YACOEL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Potassium dynamics in western uruguayan agricultural mollisols. |
Fecha de publicación : |
2017 |
Fuente / Imprenta : |
Communications in Soil Science and Plant Analysis, 30 November 2017, Volume 48, Issue 21, Pages 2558-2572. |
DOI : |
10.1080/00103624.2017.1416134 |
Idioma : |
Inglés |
Notas : |
Article history: Received 4 January 2017 // Accepted 13 November 2017. |
Contenido : |
ABSTRACT.
Knowledge of soil potassium (K) dynamics and quantification of plant-available K reserves are essential for the correct management of this nutrient. The objectives of this study, conducted in six Uruguayan mollisols, were to (i) determine the contribution of plant-available nonexchangeable K to plant nutrition, (ii) compare the ability of the ammonium acetate (NH4OAc) method and the sodium tetraphenylboron (NaBPh4) method to determine plant-available K, and (iii) quantify the effect of K fixation and release processes on the ability of both methods to estimate changes in K availability due to different K balances. In a greenhouse experiment, perennial ryegrass (Lolium perenne, cv. Horizon) was grown over a period of 320 days in six soils fertilized with 0, 100, 200, and 400 mg K kg?1. We measured plant K uptake and the changes in soil K status related to different K balances using NH4OAc and NaBPh4. Use of NaBPh4 resulted in a more accurate determination of plant-available K (R2 = 0.97 vs. 0.78) and soil K balance (R2 = 0.73 vs. 0.63), but neither of the methods was suitable for identifying positive K balances. However, when positive balances were established by K addition and incubation without plant growth, both methods related well with K balances. Again, NaBPh4 was better than NH4OAc (R2 = 0.98 and 0.88, respectively). The more accurate determination of plant K uptake and the strongest relationship with K balance of NaBPh4 was due to the extraction of exchangeable K plus a proportion of plant-available nonexchangeable K. Soil?plant interactions enhance soil K fixation in forms that are available to plant absorption but are not extractable by the chemical methods considered in this study.
© 2017 Taylor & Francis. MenosABSTRACT.
Knowledge of soil potassium (K) dynamics and quantification of plant-available K reserves are essential for the correct management of this nutrient. The objectives of this study, conducted in six Uruguayan mollisols, were to (i) determine the contribution of plant-available nonexchangeable K to plant nutrition, (ii) compare the ability of the ammonium acetate (NH4OAc) method and the sodium tetraphenylboron (NaBPh4) method to determine plant-available K, and (iii) quantify the effect of K fixation and release processes on the ability of both methods to estimate changes in K availability due to different K balances. In a greenhouse experiment, perennial ryegrass (Lolium perenne, cv. Horizon) was grown over a period of 320 days in six soils fertilized with 0, 100, 200, and 400 mg K kg?1. We measured plant K uptake and the changes in soil K status related to different K balances using NH4OAc and NaBPh4. Use of NaBPh4 resulted in a more accurate determination of plant-available K (R2 = 0.97 vs. 0.78) and soil K balance (R2 = 0.73 vs. 0.63), but neither of the methods was suitable for identifying positive K balances. However, when positive balances were established by K addition and incubation without plant growth, both methods related well with K balances. Again, NaBPh4 was better than NH4OAc (R2 = 0.98 and 0.88, respectively). The more accurate determination of plant K uptake and the strongest relationship with K balance of NaBPh4 was due to the extraction of exchangea... Presentar Todo |
Palabras claves : |
ILLITE; NONEXCHANGEABLE POTASSIUM; POTASSIUM RESERVES; SODIUM TETRAPHENYLBORON. |
Asunto categoría : |
-- |
Marc : |
LEADER 02475naa a2200205 a 4500 001 1059316 005 2018-11-28 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1080/00103624.2017.1416134$2DOI 100 1 $aNÚÑEZ, A. 245 $aPotassium dynamics in western uruguayan agricultural mollisols.$h[electronic resource] 260 $c2017 500 $aArticle history: Received 4 January 2017 // Accepted 13 November 2017. 520 $aABSTRACT. Knowledge of soil potassium (K) dynamics and quantification of plant-available K reserves are essential for the correct management of this nutrient. The objectives of this study, conducted in six Uruguayan mollisols, were to (i) determine the contribution of plant-available nonexchangeable K to plant nutrition, (ii) compare the ability of the ammonium acetate (NH4OAc) method and the sodium tetraphenylboron (NaBPh4) method to determine plant-available K, and (iii) quantify the effect of K fixation and release processes on the ability of both methods to estimate changes in K availability due to different K balances. In a greenhouse experiment, perennial ryegrass (Lolium perenne, cv. Horizon) was grown over a period of 320 days in six soils fertilized with 0, 100, 200, and 400 mg K kg?1. We measured plant K uptake and the changes in soil K status related to different K balances using NH4OAc and NaBPh4. Use of NaBPh4 resulted in a more accurate determination of plant-available K (R2 = 0.97 vs. 0.78) and soil K balance (R2 = 0.73 vs. 0.63), but neither of the methods was suitable for identifying positive K balances. However, when positive balances were established by K addition and incubation without plant growth, both methods related well with K balances. Again, NaBPh4 was better than NH4OAc (R2 = 0.98 and 0.88, respectively). The more accurate determination of plant K uptake and the strongest relationship with K balance of NaBPh4 was due to the extraction of exchangeable K plus a proportion of plant-available nonexchangeable K. Soil?plant interactions enhance soil K fixation in forms that are available to plant absorption but are not extractable by the chemical methods considered in this study. © 2017 Taylor & Francis. 653 $aILLITE 653 $aNONEXCHANGEABLE POTASSIUM 653 $aPOTASSIUM RESERVES 653 $aSODIUM TETRAPHENYLBORON 700 1 $aMORON, A. 773 $tCommunications in Soil Science and Plant Analysis, 30 November 2017, Volume 48, Issue 21, Pages 2558-2572.
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
15/08/2022 |
Actualizado : |
01/12/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
PASSOS, J.R.S.; GUERREIRO, D.D.; KAMILA S. OTÁVIO; DOS SANTOS-NETO, P.C.; SOUZA-NEVES, M.; CUADRO, F.; NUÑEZ?OLIVERA, R.; CRISPO, M.; VASCONCELOS, F.R.; BEZERRA, M.J.B.; SILVA, R.F.; LIMA, L.F.; FIGUEIREDO, J.R.; BUSTAMANTE-FILHO, I.C.; MENCHACA, A.; MOURA, A.A. |
Afiliación : |
JOSÉ RENATO S. PASSOS, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; DENISE D. GUERREIRO, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; OTÁVIO, K.S., Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; PEDRO C. DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; MARCELA SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; FEDERICO CUADRO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; RICHARD NUÑEZ?OLIVERA, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; MARTINA CRIPO, Unidad de Biotecnología en Animales de Laboratorio, Institut Pasteur de Montevideo, Montevideo, Uruguay.; FÁBIO R. VASCONCELOS, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; MARIA JULIA B. BEZERRA, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; RENATO F. SILVA, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; LARITZA F. LIMA, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; JOSÉ RICARDO FIGUEIREDO, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; IVAN C. BUSTAMANTE-FILHO, Laboratório de Biotecnologia, Universidade do Vale do Taquari, Lajeado, Brazil.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; ARLINDO A. MOURA, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil. |
Título : |
How in vitro maturation changes the proteome of ovine cumulus-oocyte complexes?. |
Complemento del título : |
Volume 89, Issue 10, Pages 459 - 470October 2022 |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Molecular reproduction and development, October 2022, Volume 89, Issue 10, pages 459-470. doi: https://doi.org/10.1002/mrd.23638 |
DOI : |
10.1002/mrd.23638 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 16 February 2022 | Accepted: 21 July 2022. -- Corresponding author: Moura, A.A.; Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Funding: The experiments presently described were conducted at the facilities of the Instituto de Reproducción Animal Uruguay (Fundacion IRAUy, Montevideo, Uruguay) and at the Unidad de Biotecnología en Animales de Laboratorio (UBAL) of the Institut Pasteur de Montevideo, Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Financial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); The Brazilian Research Council-CNPq (grants # 313160/2017-1 and 438773/2018-7); Brazilian Commission for Higher Education (CAPES); Ceará State Foundation for the Support of Technology and Scientific Development (FUNCAP), Brazil. |
Contenido : |
Abstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols for Ovis aries. © 2022 Wiley Periodicals LLC. MenosAbstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols ... Presentar Todo |
Palabras claves : |
FOLLICLE; OVARY; OVINE; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; PROTEINS; REPRODUCTION. |
Asunto categoría : |
-- |
Marc : |
LEADER 03782naa a2200409 a 4500 001 1063525 005 2022-12-01 008 2022 bl uuuu u00u1 u #d 024 7 $a10.1002/mrd.23638$2DOI 100 1 $aPASSOS, J.R.S. 245 $aHow in vitro maturation changes the proteome of ovine cumulus-oocyte complexes?.$h[electronic resource] 260 $c2022 500 $aArticle history: Received: 16 February 2022 | Accepted: 21 July 2022. -- Corresponding author: Moura, A.A.; Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Funding: The experiments presently described were conducted at the facilities of the Instituto de Reproducción Animal Uruguay (Fundacion IRAUy, Montevideo, Uruguay) and at the Unidad de Biotecnología en Animales de Laboratorio (UBAL) of the Institut Pasteur de Montevideo, Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Financial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); The Brazilian Research Council-CNPq (grants # 313160/2017-1 and 438773/2018-7); Brazilian Commission for Higher Education (CAPES); Ceará State Foundation for the Support of Technology and Scientific Development (FUNCAP), Brazil. 520 $aAbstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols for Ovis aries. © 2022 Wiley Periodicals LLC. 653 $aFOLLICLE 653 $aOVARY 653 $aOVINE 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL 653 $aPLATAFORMA DE SALUD ANIMAL 653 $aPROTEINS 653 $aREPRODUCTION 700 1 $aGUERREIRO, D.D. 700 1 $aKAMILA S. OTÁVIO 700 1 $aDOS SANTOS-NETO, P.C. 700 1 $aSOUZA-NEVES, M. 700 1 $aCUADRO, F. 700 1 $aNUÑEZ?OLIVERA, R. 700 1 $aCRISPO, M. 700 1 $aVASCONCELOS, F.R. 700 1 $aBEZERRA, M.J.B. 700 1 $aSILVA, R.F. 700 1 $aLIMA, L.F. 700 1 $aFIGUEIREDO, J.R. 700 1 $aBUSTAMANTE-FILHO, I.C. 700 1 $aMENCHACA, A. 700 1 $aMOURA, A.A. 773 $tMolecular reproduction and development, October 2022, Volume 89, Issue 10, pages 459-470. doi: https://doi.org/10.1002/mrd.23638
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